Laboratory Objectives Summer 2025 Tuesday or Thursday at 12:30 on Campus Room Science 2089
Instructor: Dr. Clare Hays, SI 2032; 303-615-0777, e-mail – [email protected], URL http://sites.msudenver.edu/haysc
REQUIRED Books and Supplies
- Your textbook is for lecture, but doesn’t need to come to school with you: Seeley’s Anatomy & Physiology, Evergreen Ed., by Van Putte, Regan, Russo;
2. Your lab manual needs to come to lab with you: Human Anatomy and Physiology Laboratory Manual, Cat Version, 13th Ed., Elaine N. Marieb. Pearson+ eText of Marieb, Human A+P Lab Manual w/ Cat Dissections, 13e
3. Dissecting Tools. Available in bookstore; includes a scalpel with replaceable blades, a blunt probe, and small scissors;
4. Familiarize yourself with the safety rules for lab and dissection protocols.
OPTIONAL or RECOMMENDED Supplies and Resources
1. Not required, but strongly recommended, is a lab coat or an old shirt to protect your clothing. Respirators with filters and eye goggles are available upon request.
2. Upon completion of lab exercises, you may review the material and do the review sheets from your lab manual, as there are no open lab hours. The review sheets are helpful but are not turned in.
3. Your lab manual has some excellent resources for both lecture and lab. They are found at masteringaandp.com, but an access code is needed. These resources and the access code are described at the beginning of your lab manual. You will need to complete a registration process to use this site by clicking you are a student. Then, click Register for Self-Study Access Only and “Mastering is not required for my course.” Enter your access code and click on your book. Go to the Study Area, especially note the PAL section on anatomy.
4. Histology Reference: BIO 2320 Histology Resource
EXTRA CREDIT OPPORTUNITY: Attending lab is essential to success in the class, as it provides visual and tactile input to the structures you need to know as well as relationships of one structure to another. You may earn extra credit points for attending labs throughout the semester as follows, provided that you are not more than 20 minutes late to any given lab:
Attending fewer than 2 labs: 0 points extra credit
Attending 2 labs: 5 points extra credit
Attending 3 labs: 10 points extra credit
Attending 4 labs: 15 points extra credit
Attending 5 labs: 20 points extra credit
Attending all 6 in-person labs: 25 points extra credit
JUNE 10,12 – LANGUAGE OF ANATOMY AND ENDOCRINE ANATOMY
LANGUAGE OF ANATOMY:
Exercise 1: Glance at Figure 1.2 to understand anatomic terminology of the quadruped (dog or cat). Use your own body and the human torso models to refresh on the basic organization of the body. Know the following terms:
Anatomical Position (both human and quadruped), Superior, Inferior, Cranial, Caudal, Medial, Lateral, Superficial, Deep, Ventral, Anterior, Dorsal, Posterior, Proximal, Distal, Sagittal plane, Transverse plane, Frontal plane, Thoracic cavity, Abdominal cavity, Pelvic cavity.
ENDOCRINE ANATOMY:
Exercise 27: Figure 27.1 in the Marieb lab manual has human endocrine pictures and microscopic anatomy.
Exercise 3 Cat Dissection in back of lab manual: Obtain a cat and open the ventral body cavity by cutting through linea alba on the abdomen and then using scissors to cut through the ribcage on the ventral side of the cat, but slightly off center as described in your lab manual. Be careful not to remove the gonads and do not cut through blood vessels containing colored latex, without first checking with your instructor. Additionally, a fatty greater omentum covers all of the abdominal visceral. You may peel it out of your way, but do not remove it.
- Observe the following endocrine glands of the cat: Thyroid, Thymus, Pancreas, Adrenal, Ovary, Testis.
- Observe the pituitary gland and pineal gland (=body) on the preserved sheep brain. See Figures 27.1 and 27.2. Sheep pineal picture in transverse fissure. Sheep pineal picture from sagittal view.
- Put your cat away as described by your instructor. Clean your working area thoroughly.
- Observe the microscopic anatomy on the Thyroid gland, Pancreas, Adrenal gland, Ovary, and Testis as described in Exercise 27 – Activity 2 of lab manual as well as Figures 42.2 and 43.6. Refer to #4 under Recommended Resources listed above for histology tips.
JUNE 17, 19 – BLOOD and HEART
BLOOD:
Exercise 29: Complete the following activities using sheep blood or fake blood.
Activity 1: Observe the color and clarity of plasma after you conduct the hematocrit test (to be done later in this lab).
Activity 2: Observe one of each formed elements on a prepared human blood sample slide. You must be able to identify erythrocytes, thrombocytes, and each of the granulocytes (neutrophils, eosinophils, basophils) and each of the agranulocytes (lymphocytes and monocytes). Note: All granulocytes and agranulocytes are types of leukocytes. (Practice your blood cell identification with the pictures in your Week Two Lab Module in Canvas.)
Activity 4: Conduct a Hematocrit using the microhematocrit reader card. Then, observe the color and clarity of plasma from Activity 1. See if you can spot the layer of leukocytes found in the buffy coat between the plasma and the red blood cells.
Activity 5: Determine the approximate hemoglobin concentration of the blood sample using the Tallquist method.
Activity 7: Obtain an unknown blood sample and conduct the blood typing experiment to determine its ABO and Rh factor.
ANATOMY OF THE HEART:
Exercise 30: Use Figures 30.2, 30.3, 30.4, 30.7, 30.8 for your heart anatomy.
Observe the sheep heart which has been cut in a frontal section. You are responsible for the following structures:
Visceral pericardium (epicardium), myocardium, endocardium, coronary blood vessels, left and right atria, left and right ventricles, auricles, pulmonary trunk, aorta, aortic semilunar valve, pulmonary veins, superior and inferior vena cavae, right atrioventricular valve (tricuspid), pulmonary semilunar valve, interventricular septum, papillary muscles, chordae tendineae, and left atrioventricular valve (bicuspid).
Mediastinum, pericardial sac, and pericardial cavity are best observed on your cat. Follow instructions from your instructor.
Observe the microscopic anatomy of cardiac muscle as described in Exercise 30 – Activity 4 and Figure 30.6.
Here is a good heart overview video made by Sophie, a previous TA.
JUNE 24, 26 – BLOOD VESSELS
Exercise 4 Cat Dissection: a) Dissect your cat as described in Dissection Exercise 4 in back of lab manual. You are responsible for the following blood vessels:
Vessels Cranial to Diaphragm: Aorta, Coronary arteries, superior vena cava (precava), inferior vena cava (postcava), pulmonary trunk (arteries), pulmonary veins.
Azygos vein.
Brachiocephalic veins, external jugular veins, subclavian veins, axillary veins, brachial veins, cephalic veins.
Brachiocephalic artery, left subclavian artery, right subclavian artery, common carotid arteries, external carotid arteries, vertebral arteries, axillary arteries, brachial arteries. (note: both common carotids come off of the brachiocephalic artery in cats, but in humans, the left common carotid actually comes off of the aortic arch)
Vessels Caudal to Diaphragm: Hepatic veins, adrenolumbar veins (=suprarenal veins), renal veins, testicular or ovarian veins, iliolumbar veins, common iliac veins, internal iliac veins, external iliac veins, femoral vein, great saphenous vein, popliteal vein.
Hepatic portal vein, gastrosplenic vein, superior mesenteric vein, inferior mesenteric vein. (These vessels are prepared with yellow dye.)
Aorta, celiac trunk, left gastric artery, hepatic artery, splenic artery, superior mesenteric artery, adrenolumbar arteries (=suprarenal arteries), renal arteries, testicular or ovarian arteries, inferior mesenteric artery, iliolumbar arteries, external iliac arteries (note: cats do not have a common iliac artery as humans do), internal iliac arteries, femoral artery, popliteal artery.
This video shows human and cat vessels.
JUNE 30 through JULY 5 – EXAM 1
(25 questions, 50 points)
LAB EXAM ONE You do not come to lab this week, as the exam is on Canvas. The online lab test is primarily fill in the blank questions randomly selected from a test bank and spelling must be exact. It may be found on MSU Denver Canvas. It opens June 30 at 12 am and closes at 11:59 pm on Saturday July 5. The online tests have 25 questions at 2 points each, 30 minutes. The exam will only test you on material from these laboratory objectives (Anatomy Language, Endocrine, Blood, Heart and Blood Vessels). I do put my eyes on everyone’s completed exams to double check the computer grading. Read Exam Directions:
- All answers for fill-in-the-blank questions are in lower case letters and must be spelled correctly.
- You never have to indicate right or left unless it is a heart chamber.
- You will need to specify artery or vein unless that word is given in the question.
Any lab exam not taken by 11:59 pm on Saturday July 5 will have a 10% deduction per day late.
JULY 8, 10 – CARDIOVASCULAR PHYSIOLOGY (Both Exercises 31 and 33)
Exercise 31: Understand what is recorded with an electrocardiogram (ECG or EKG) and where leads are placed for a Lead II EKG. On the Lead II EKG, identify the P wave, QRS complex, and the T wave. Understand what events are taking place during these 3 recognizable waves. Observe abnormal EKGs and the important information that can be gleaned on heart pathology from these. However, you will only be tested on normal EKGs.
INTERPRETATION OF ELECTROCARDIOGRAM: The P wave represents atrial depolarization and the QRS complex represents ventricular depolarization. The T wave represents ventricular repolarization. Atrial repolarization is not visible, as it occurs during the dominant QRS complex.
Exercise 33: Complete the following activities for heart sounds, blood pressure and pulse determinations.
Activity 1: Complete Auscultating Heart Sounds.
Activity 2: Palpate Superficial Pulse Points.
Activity 4: Taking an Apical Pulse.
Activity 5: Use a sphygmomanometer to measure arterial blood pressure.
Activity 6: Estimate your venous pressure, both at rest and while performing the Valsalva maneuver.
Activity 7: Observe the effect of posture, exercise and ice water on blood pressure and heart rate.
Activity 8: Observe the effect of local chemical and physical factors on skin color.
JULY 15, 17 – ANATOMY OF RESPIRATORY, DIGESTIVE, URINARY and REPRODUCTIVE SYSTEMS
RESPIRATORY ANATOMY:
Exercise 36: Examine a microscopic section of lung tissue as described on p. 538. Refer to #4 under Recommended Resources listed above for histology tips.
Exercise 6 Cat Dissection: Dissect your cat as described on Cat Dissection Exercise 6. You are responsible for:
External nares, Oral cavity, oropharynx (oral pharynx), trachea, larynx, thyroid cartilage, cricoid cartilage, epiglottis, hyoid bone, vagus nerve, primary bronchi, pleural cavities, parietal pleura, visceral pleura, diaphragm, phrenic nerve, and lungs.
DIGESTIVE ANATOMY:
Exercise 38: Observe microscopic sections of the stomach, small intestine, pancreas, liver, colon and taste buds. See Figures 38.6, 38.9, 38.16, 26.3, 27.3c. Refer to #4 under Recommended Resources listed above for histology tips.
Exercise 7 Cat Dissection: Dissect your cat as described on Dissection Exercise 7. You are responsible for:
Parotid salivary gland, teeth, hard palate, soft palate, tongue papillae, frenulum of tongue, esophagus, parietal peritoneum, liver, greater omentum, gall bladder, stomach [cardia, fundus, body, pylorus], greater and lesser curvature of stomach, lesser omentum, pancreas, spleen, common bile duct, small intestine [duodenum, jejunum, ileum], mesentery proper, cecum, colon [ascending, transverse, descending], rectum, anus, and visceral peritoneum.
URINARY and REPRODUCTIVE ANATOMY:
URINARY ANATOMY:
a) Exercise 40: Observe the following structures on a cat kidney: Renal capsule, cortex, medulla, medullary pyramids, and renal pelvis.
b) Dissect your cat as described on Cat Dissection Exercise 8. You are responsible for: Kidneys, hilus (=hilum), ureter, urinary bladder, and urethra.
REPRODUCTIVE ANATOMY:
Exercise 9 Cat Dissection: You are responsible for the anatomy of BOTH male and female cats. Dissect as described on Dissection Exercise 9 and know the following:
a) Penis, scrotum, testes, spermatic cord, ductus deferens (=vas deferens), inguinal canal, prostate gland.
b) Uterus (uterine body & 2 uterine horns), uterine tube (=fallopian tube=oviduct), ovary, vagina, cervix, and vulva.
c) Observe the microscopic anatomy of the ovary and testis. Refer to Figures 42.2 and 43.6. Refer to #4 under Recommended Resources listed above for histology tips.
JULY 22, 24– RESPIRATORY PHYSIOLOGY, URINALYSIS, MOVIE
RESPIRATORY PHYSIOLOGY:
Exercise 37: Complete the following physiology activities.
Activity 2: Auscultating Respiratory Sounds using a stethoscope.
Activity 3: Measuring Respiratory Volumes using a Non-Recording Wet Spirometer
One person from each group should be the subject and complete #1-7 in your lab manual. Measurements should be recorded for the following
- Respiratory Rate (RR)
- Tidal Volume (TV)
- Minute Respiratory Volume (TV X RR)
- Expiratory Reserve Volume (ERV)
- Vital Capacity (VC)
- Inspiratory Reserve Volume (IRV) Use the calculation IRV = VC – (TV + ERV)
Activity 6: Complete this activity without using a spirometer. Determine the main regulator of breathing.
URINALYSIS and REPRODUCTIVE PHYSIOLOGY FILM:
Exercise 41: Analyze a urine sample from your own urine and from an unknown provided. Interpret your results.
Activity 1: Complete as much of the Urinalysis Results form as possible using information derived from observation, reagent strips, and the refractometer.
Activity 2: Complete a sediment analysis on your own urine as described.
A one-hour film on reproduction, Miracle of Life, may be viewed at home. There will be simple exam questions on the film (meaning watch it, but no need to take notes). Here is a link to the film: https://archive.org/details/NOVATheMiracleOfLife
JULY 28 through AUGUST 2 – EXAM 2
(25 questions, 50 points)
LAB EXAM TWO You do not come to lab this week, as the exam is on Canvas. The online lab test is primarily fill in the blank questions randomly selected from a test bank and spelling must be exact. It may be found on MSU Denver Canvas. It opens July 28 at 12 am and closes at 11:59 pm on Saturday August 2. The online tests have 25 questions at 2 points each, 30 minutes. The exam will only test you on material from these laboratory objectives (CV Phys, Resp & Dig & Uri & Repro anatomy, Resp phys, Urinalysis). I do put my eyes on everyone’s completed exams to double check the computer grading. Read Exam Directions:
- All answers for fill-in-the-blank questions are in lower case letters and must be spelled correctly.
- You never have to indicate right or left.
No late lab exams, because the semester ends on Saturday August 2.